Pharmacy Department/School of Health Care Professions - University of San Carlos
Sesuvium portulacastrum Linn (1753) plant extracts were subjected to a toxicity screening. Brine Shrimp Lethality Assay and Allium cepa Chromosome Aberration Assay was conducted to assess the plant's toxicity. In addition, a phytochemical screening was also conducted to determine the presence of secondary metabolites.
Brine Shrimp Lethality Assay showed that the plant extract was toxic. At 36mg/mL and 60mg/mL concentration, the extract from maceration proved to be the most toxic, with the LC50 of 10.60mg/mL. The least toxic was the extract from decoction at 42mg/mL with the LC50 of 92.99mg/mL. Allium cepa Chromosome Aberration Assay showed that the plant extract possess genotoxic activity as demonstrated by the presence of bridges, fragments, laggards and vagrants. It also showed that the plant extract was able to inhibit root growth. The plant extract from maceration showed the greatest inhibitory effect. At 60mg/mL concentration, the plant extract was able to inhibit 100%, had the most number of aberrations and had the least number of dividing cells.
The phytochemical screening conducted revealed the presence of the constituents of the plant extract. The extract from decoction tested positive for reducing sugar, saponin and resin. The extract from maceration tested positive for reducing sugar, saponin and tannins. The general toxicity of the plant extract can be attributed to the presence of these secondary metabolites.
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