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In vitro antiurolithiatic activity of Busikad Cyperus kyllingia (ROTTB, 1842) leaf extract.


Related Institution

Pharmacy Department/School of Health Care Professions - University of San Carlos

Publication Information

Publication Type
Thesis Degree
Publication Date
March 2018


     This study dealt with the determination of the antiurolithiatic activity of the ethanolic extract of fresh Cyperus kyllingia (Busikad) leaves. The plant is widely distributed as a weed and can easily be found in fresh soil. The constituents of the fresh leaves were extracted by maceration using 95% ethanol. The green extract was heated to dryness and underwent phytochemical screening which showed positive results for phenol and flavonoids, known to be the cause of its antiurolithiatic activity. Confirmation of antiurolithiatic activity was done using three methods - Nucleation assay, Aggregation assay and Dissolution Assay which target the primary steps of stone formation as well as dissolution of stone. In Nucleation assay, treatment groups D had the highest inhibition at 89.12±4.73. Aggregation assay had the highest inhibition at 85.11%±4.23. Dissolution assay had highest activity with treatment D at 87.19%±3.66. Results from the tests showed an increase inhibitory activity with increasing concentrations. The median inhibitory concentration of Cyperus kyllingia leaf extract for nucleation and aggregation was determined using linear regression analysis. The IC50 of the extract for crystal nucleation is 0.2831 mg/ml. The IC50 of crystal aggregation is 0.8056 mg/ml indicating that at this concentration the plant can have a fifty percent (50%) inhibitory activity. The median effective concentration of Cyperus kyllingia leaf extract for crystal dissolution was also determined using linear regression analysis. The EC50 of the leaf extract for crystal dissolution is 1.0132 mg/ml indicating a fifty percent (50%) effectivity at this concentration.


1. Ahmed, Salman , Hsan, Muhammad . "In vitro urolithiasis models: An evaluation of prophylactic management against kidney stones" Journal of Pharmacognosy and Phytochemistry , 28-35, 2016
2. Badmus, J A, Odunola, O A, Obuotor , E M, Oyedapo, . "Phytochemicals and in vitro antioxidant potentials of defatted methanolic extract of Holarrhena floribunda leaves" African Journal of Biotechnology , 2010
3. Bawane, Praddep , Jain, Meeta , Gawad, Jineetkumar , Godad, Angel , Tauro, Sativa . "Evaluation of polyherbal extracts for in-vitro Antiurolithiatic Activity using Bioenhancer" European Journal of Biomedical and Pharmaceutical Sciences , 480-488, 2014
4. Byahatti, Vivek , Vasantakumar Pai , , D’Souza., Marina . "Effect of Phenolic Compounds from Bergenia ciliata (Haw.)Sternb. leaves on Experimental kidney stones" Ancient Science of Life , 14-17, 2010
5. Scott, C , Nishimoto, R K, Tang, C S. "Cyperus kyllingia and Cyperus brevifolius: a potential model for the study of allelopathy" HortScience , June 1992
6. Devkar, Rafiraj , Chaudhary, Shilpee , Adepu, Sahithi . "Evaluation of antiurolithiatic and antioxidant potential of Lepidagathis prostrate: A Pashanbhed plant" Pharmaceutical Biology , 1239-1240, 2016
7. Saravanasingh, K , Ramamurthy, M , Parthiban, P . "In-vitro Anti-Urolithiatic Activity of Aerial partsof Aerva lanata (L.) Juss" International Journal of Current Research in Medical Sciences , 24-27, 2016
8. Finkielstein, Vadim , Goldfarb, David . "Strategies for preventing calcium oxalate stones" CMAJ-JAMC , 1407-1049, 2006
9. Mohamed, Gamal , Kamal, M , Hani, K , Ibrahim, R , Sabrin, M . "Compounds Isolated from Cyperus Species (Part II): terpenoidal" International Journal of Pharmacognosy and Phytochemical Research , 83-99, 2015
10. Garcia-Perdoma, Paola Benavidez Sola , Espana, Paola Posada . "Pathophysiology associated with forming urinary stones" Reicta Urologia Colombiana , 118-125, October 2015
11. Geetha, N , Saranya, R . "Inhibition of Calcium Oxalate (coax) Crystallization In Vitro by the Extract" International Journal of Pharmacy and Pharmaceutical Sciences , 2014
12. Mert, Ilhan , Ergene, Burcin , Ipek, Suntarm . "Preclinical Evaluation of Antiurolithiatic Activity of Viburnum opulus L. on Sodium Oxalate-Induced Urolithiasis Rat Model" Evidence-Based Complementary and Alternative Medicine , 52-62, 2014
13. Jenny, Anowara , Saha, Dibyajyoti , Paul, Swati , Dutta, Mycal , Uddin, Zia , Nath, Aninda Kumar . "Antibacterial activity of aerial part of extract of elephantopus scaber linn" Bulletin of Pharmaceutical Research , 38-41, 2012
14. Saravanasingh, K , Ramamurthy, M , Parthiban, P . "In-vitro Anti-Urolithiatic Activity of Aerial parts of Aerva lanata (L.)  Juss" International Journal of Current Research in Medical Sciences 2(3): 26-27, March 2016
15. Khare, Prachi , Mishra, Vinod , Arun, Kakkar , Bais, Neetu . "Study on In vitro anti-lithiatic activity of Phyllantus niruri linn. Leaves byhomogenous precipitation and turbidity method" International Journal of Pharmacy and Pharmaceutical Sciences , 124-127, 2014
16. Knoll, Thomas . "Epidemiology, Pathogenesis, and Pathophysiology of Urolithiasis" European urology supplements , 802-806, 2010
17. Lopez, M , Hoppe, B . "History,epidemiology and regional diversities of urolithiasis" Pediatric Nephrology (Berlin, Germany) 25(1): 49-59, 2001
18. Mamillapalli, Vami , et al., . "Phytochemical & in vitro antiurolithiatic studies on the leaf extracts of Bauhinia variegata linn." International Journal of Pharmaceutical Sciences and Research , 2015
19. Mohamed, Gamal , Elhady, Sameh , Ibrahim, Sabrin . "Compounds Isolated From Cyperus Species (Part I): Phenolics and Nitrogenous" International Journal of Pharmacognosy and Phytochemical Research , 51-67, 2015
20. Rathod, N , Chitme, H R, Chandra, R . "In vivo and In vitro models for evaluating Anti-urolithiasis activity of Herbal drugs" International Journal of Pharmaceutical Research and Bio-Science , 309-329, 2014
21. Jaiswal , Nikita , Singh, Shipra . "Ethnobotany and Diuretic activity of some selected medicinal plants" The Journal of Phytopharmacology 1(2): 21-28, 2012
22. Robertson, W G. "Pathophysiology of Stone Formation" Karger , 329-333, 2016
23. Ratnam, K V, et., . "Evaluation of diuretic and antiurolithiatic activities of ethanolic root extract of curculigo orchiodes" International journal of biological and pharmaceutical research , 1-5, 2013
24. Singh, S.D. J, Krishna, V , Mankani, K L, Manjunatha, B K, Vidya, S M, Manohara, Y N. "Wound Healing activity of the leaf extracts and deoxyelephantopin isolated from Elephantopus scaber Linn." Indian Journal of Pharmacognosy , 238-242, 2005
25. Singh, O. Ibopishak , Devi, A. Bimola . "Comparative study of the Antiurolithiatic Activity of Plant Hydroalcoholic Extract of Argemone Mexican, Cissus javana DC and Garcinia pendunculata" International Journal of Science and Research (IJSR) , 2066-2070, 2013
26. Saha, Sarmistha , Verma, Ramtej J.. "Inhibition of calcium oxalate crystallisation in vitro by an vitro by anextract of Bergennia ciliata" Arab Journal of Urology (AJU) , 187-192, 2013
27. Usman, H , Abdulrahman, F I, Usman, U . "Qualitative Phytochemical Screening and In Vitro Antimicrobial Effects of Methanol Stem Bark Extract of Ficus Thonningii (Moraceae)" African Journal of Traditional, Complementary and Alternative Medicines (AJTCAM) , 289-295, 2009
28. Vyas, B A, Joshi, S V, Santani, D D. "Antiurolithiatic Activity of Whole-Plant Hydroalcoholic Extract of Pergularia daemia in Rats" Elsevier , 36-40, 2011
29. VanMeter, Karin C., Hubert, Robert J.. Pathophysiology for the Health Professions Elsevier Health Sciences, 2013.
30. McNutt, William Fletcher . Diseases of the Kidneys and Bladder J.B. Lippincott, 1893.
31. Smith, J. Kevin . Urinary Calculi Imaging
32. Christian, Turk , et. al., . EAU Guidelines on Diagnosis and Conservative Management of Urolithiasis European Urology, 468-474. (Vol 69 no.)
33. Dave, Chirag . Nephrolithiasis 2016.
34. (). Anuang. Wikipilipinas: The Hip n’ Free Philippine Encyclopedia. . Retrieved from: http://en.wikipilipinas.org/index.php/Anuang
35. (). Anuang, Kyllinga monocephala Rottb., Whitehead spikehedge, Dan sui shui wu gong. Philippine Medicinal Herbs/Alternative Medicine. . Retrieved from: http://www.stuartxchange.org/Anuang.htm
36. (2016, November 30). Health Grove. . Retrieved from: http://global-disease-burden.healthgrove.com/1/69411/Urolithiasis-in-The-World

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